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2016.09.26 14:10

[Thu. 29 Sep. 2016] Prof. Tae-Young Yoon/Observation of Single Membrane Proteins under Mechanical Tension

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Speaker Prof. Tae-Young Yoon
Date Thursday 29 September 2016
Time 5:00pm
Venue #331, Asan Hall, College of Science

“Observation of Single Membrane Proteins

under Mechanical Tension”

 

 

Single-molecule methods, which handle and monitor molecules one at a time, have been a powerful toolkit for elucidating molecular mechanisms underlying many fundamental biological processes. I will talk about two recent efforts made in our lab, where we endeavor to apply single-molecule methods to a special class of proteins, that is, membrane proteins. First, by applying pN-scale force to a single SNARE complex, a main force-generating machine for membrane fusion in all eukaryotes, we reveal large hysteresis in a mechanical unizipping and rezipping cycle of the SNARE complex. With combined application of different single-molecule methods, we show how this rigid SNARE complex is efficiently disassembled by NSF and SNAP. We observed that NSF exploits a spring-loaded mechanism to tightly couple its ATP hydrolysis with unfolding of the SNARE complex. Second, by employing single-molecule fluorescence imaging as the detection scheme, my lab is developing a single-molecule version of the co-immunoprecipitation (co-IP) analysis. Harnessing its single-molecule sensitivity and millisecond time resolution, we quantitatively determine protein-protein interactions and their relevant changes in given cells or tissues. This novel capability will shed light onto the molecular lesions that drive individual cancers at the protein-protein interaction level, where signal transduction physically occurs. I will close the talk with brief outlook for future researches.

 

Prof. Tae-Young Yoon
Center for Nanomedicine, Institute for Basic Science (IBS), Yonsei University, Seoul, South Korea​


List of Articles
No. Subject Speaker Date
Notice Fall semester 2017 Seminar Schedule file - -
29 [Thu. 1 Jun. 2017] Prof. Jong-Man Kim/Department of Chemical Engineering, Hanyang University, Seoul, Korea Prof. Jong-Man Kim Thu. 1 Jun. 2017
28 [Thu. 25 May. 2017] Prof. Wonsuk Kim/Department of Chemistry and Nano Science, Ewha Womans University, Seoul Prof. Wonsuk Kim Thu. 25 May. 2017
27 [Thu. 18 May. 2017] Student Host Colloquium : 1,1-Bisborylalkane: New Class of Organoboron Compound for the Chemo, Regio and Stereoselective Organic Transformations Prof. Seung Hwan Cho Thu. 18 May. 2017
26 [Thu. 11 May. 2017] Prof. Andreas Heinrich/IBS Research, Center for Quantum Nanoscience, Seoul, South Korea Prof. Andreas Heinrich Thu. 11 May. 2017
25 [Thu. 27 Apr. 2017] Seminar was canceled! - -
24 [Thu. 13 Apr. 2017] Dr. Wan Ki Bae/Photoelectronic Hybrid Research Center, Korea Institute of Science and Technology, Seoul, Korea Dr. Wan Ki Bae Thu. 13 Apr. 2017
23 [Thu. 6 Apr. 2017] Prof. Rong-Jie Chein/Institute of Chemistry, Academia Sinica, Nankang, Taipei, Taiwan Prof. Rong-Jie Chein Thu. 6 Apr. 2017
22 [Thu. 30 Mar. 2017] Prof. Jeong Ho Cho/SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon, Korea Prof. Jeong Ho Cho Thu. 30 Mar. 2017
21 [Thu. 23 Mar. 2017] Prof. Sangsu Bae/Department of Chemistry, Hanyang University, Seoul, South Korea Prof. Sangsu Bae Thu. 23 Mar. 2017
20 [Fri. 17 Mar. 2017] 9th RINS Symposium file - Fri. 17 Mar. 2017
19 [Thu. 9 Mar. 2017] Prof. Woon Ju Song/Department of Chemistry, Seoul National University, Seoul, Korea Prof. Woon Ju Song Thu. 9 Mar. 2017
18 Spring semester 2017 Seminar Schedule file - -
17 [Mon. 9 Jan. 2017] Prof. Sungwook Woo/Wyss Institute for Biologically Inspired Engineering, Harvard University, USA Prof. Sungwook Woo Mon. 9 Jan. 2017
16 [Wed. 28 Dec. 2016] Prof. James Y. Hamilton/Development of a Broad Range of Asymmetric C–C Bond Forming Reactions with a Versatile Iridium Catalyst file Prof. James Y. Hamilton Wednesday 28 December 2016
15 [Thu. 1 Dec. 2016] Prof. Sang-Yong Ju/Highly Pure Carbon Nanotubes sorted by a Flavin for Thin Film Transistor Applications Prof. Sang-Yong Ju Thursday 1 December 2016
14 [Thu. 17 Nov. 2016] Prof. Seunghoon Shin/Synthetic Methods Development based on Rational Analysis of Intermediates file Prof. Seunghoon Shin Thursday 17 November 2016
13 [Thu. 3 Nov. 2016] Prof. Min-Sik Kim/Mass spectrometry and its applications to Biological Discovery Prof. Min-Sik Kim Thursday 3 November 2016
12 [Thu. 20 Oct. 2016] Prof. Chan Hyuk Kim/Novel Small Molecule-based Approaches for Cancer Immunotherapy Prof. Chan Hyuk Kim Thursday 20 October 2016
11 [Mon. 13 Oct. 2016] Prof. Amir H. Hoveyda/Multicomponent Catalytic Synthesis of Organoboron Compounds for Scalable Natural Product Synthesis Prof. Amir H. Hoveyda Monday 13 October 2016
10 [Thu. 6 Oct. 2016] Prof. Young S. Park/Atomistic Molecular Engineering of Polycyclic Aromatic Hydrocarbons Prof. Young S. Park Thursday 6 October 2016
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