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[March 28. 2019] NMR Structural Studies of engineered novel antimicrobial peptides

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Speaker Prof. Yongae Kim (Hankuk University of Foreign Studies)
Date Thu. 28 Mar. 2019
Time 5:00pm
Venue #331, Asan Hall, College of Science

NMR Structural Studies of engineered novel antimicrobial peptides

 

  Since the discovery of penicillin, antibiotics have brought benefits such as prolonging human life, treating bacterial infections as well as promoting animal growth, but misuse and abuse of antibiotics have caused serious public health problems and economic losses. Thus, antibiotics that work in a completely different way from conventional antibiotics have become necessary, and long-term solutions to these problems include antimicrobial peptides produced by all organisms, from bacteria to vertebrates and invertebrates.

  We have successfully identified the structure and activity of Lactophoricin (LPcin), an antimicrobial peptide found in bovine milk, through expression using recombinant DNA technology, purification using various biophysical techniques, and structural studies using liquid/solid-state NMR [1, 2]. Based on this LPcin, we constructed a total of 20 analogs library through conservative sequence modification to develop new antimicrobial peptides with shorter length and improved antimicrobial activity than conventional LPcin. Among them, six new antimicrobial peptides were selected through bacterial killing assays and growth inhibition assays, and each peptide was obtained a high purity, sufficient amount for structural studies by optimization of the expression and purification process [3, 4]. In addition, each peptide identified its potential as a new antibiotic through hemolysis assay and cytotoxicity assay, and to investigate the relationship between active-structure by conducting a 3D topology structure study using liquid/solid-state NMR. 

 

[1] Park TJ., Kim JS., Ahn HC. and Kim Y., “Solution and Solid-State NMR Structural Studies of Antimicrobial Peptides LPcin-I and LPcin-II”, Biophysical Journal, Vol. 101, (2011), pp 1193–1201.

[2] Jeong JH, Kim JS, Choi SS. and Kim Y., “NMR Structural Studies of Antimicrobial Peptides: LPcin Analogs”, Biophysical Journal, Vol. 110, (2016), pp 423–430.

[3] Kim JS., Jeong JH. and Kim Y., “Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin” J. Microbiol. Biotechnol. Vol. 27, (2017), 759–767

[4] Kim Y., Kim JS., Jeong JH., “Design and NMR Structural Studies of new Antimicrobial Peptides with higher activity” Biophysical Journal, Vol. 114, (2018), pp 159a.

 

 


List of Articles
No. Subject Speaker Date
Notice Spring semester 2019 Seminar Schedule file - -
58 [April 4. 2019] Rational Design of Solid-State Materials with Asymmetric Coordination Environment Prof. Kang Min Ok (Sogang University) Thu. 4 Apr. 2019
» [March 28. 2019] NMR Structural Studies of engineered novel antimicrobial peptides Prof. Yongae Kim (Hankuk University of Foreign Studies) Thu. 28 Mar. 2019
56 [March 21, 2019] Nanomaterials Design for Energy Conversion and Storage Prof. Jong-Hyuk Park (Yonsei University) Thu. 21 Mar. 2019
55 [March 27, 2019] Hyperpolarization Researches with NV center, 129Xe and Parahydrogen file Prof. Keunhong Jeong March 27, 2019
54 [March 20, 2019] Challenges in molecular dynamics simulations of unfolded proteins and protein-DNA complexes file Dr. Jejoong Yoo March 20, 2019
53 [March 5. 2019] Innovation@Agilent file Dr. Darlene Solomon (Agilent Technologies Inc.) Tuesday. 5 March. 2019
52 [March 5. 2019] Prof. Michael J. Sailor/Treatment of S. aureus Bacterial Infections with Porous Silicon Nanoparticles Prof. Michael J. Sailor (UC San Diego) Tuesday. 5 March. 2019
51 [Thu. 6 Dec. 2018] Prof. Dong-Ho Kim/Department of Chemistry, Yonsei University, Seoul, Korea Prof. Dong-Ho Kim (Yonsei University) Thu. 6 Dec. 2018
50 Fall semester 2018 Seminar Schedule file - -
49 [January 24, 2018] Electro-Optic Organic THz Wave Sources file Prof. O-Pil Kwon(Ajou University) January 24, 2018 (Wednesday)
48 [Thu. 30 Nov. 2017] Prof. Sunmin Ryu/Department of Chemistry, POSTECH, Pohang, South Korea Prof. Sunmin Ryu Thu. 30 Nov. 2017
47 [Thu. 23 Nov. 2017] Prof. Sung-Gon Kim/Department of Chemistry, Kyonggi University, Suwon, Korea Prof. Sung-Gon Kim Thu. 23 Nov. 2017
46 [November 14, 2017] Zero-Point Energy, Fluctuations, and Dissipation : A (Mostly) Quantum-Optical Perspective Prof. Milonni, Peter November 14, 2017
45 [Thu. 16 Nov. 2017] Prof. Hyungjun Kim/Department of Chemistry, and Graduate school of Energy, Environment, Water, and Sustainability (EEWS), KAIST, Daejeon, Korea Prof. Hyungjun Kim Thu. 16 Nov. 2017
44 [Fri. 30 Oct. 2017] Prof. Igor Rodin/Department of Chemistry, Moscow State University, Moscow, Russia Prof. Igor Rodin Fri. 30 Oct. 201
43 [October 31, 2017] New ways of vision: Protein structures in translational medicine and business development, my experience file Prof. Robert Huber(1988 Nobel Prize in Chemistry) October 31, 2017
42 [Thu. 26 Oct. 2017] Dr. Jung-Nyoung Heo/Center for Medicinal Chemistry Research, Korea Research Institute of Chemical Technology, Daejeon, Korea Dr. Jung-Nyoung Heo Thu. 26 Oct. 2017
41 [October 25, 2017] Ultrafast electron microscopy: applications in chemical and materials sciences Dr. Byung-Kuk Yoo October 25, 2017
40 [Mon. 16 Oct. 2017] Prof. Kirk S. Schanze/Department of Chemistry, University of Texas at San Antonio, Texas, USA Prof. Kirk S. Schanze Mon. 16 Oct. 2017
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