|Speaker||Prof. Yongae Kim (Hankuk University of Foreign Studies)|
|Date||Thu. 28 Mar. 2019|
|Venue||#331, Asan Hall, College of Science|
NMR Structural Studies of engineered novel antimicrobial peptides
Since the discovery of penicillin, antibiotics have brought benefits such as prolonging human life, treating bacterial infections as well as promoting animal growth, but misuse and abuse of antibiotics have caused serious public health problems and economic losses. Thus, antibiotics that work in a completely different way from conventional antibiotics have become necessary, and long-term solutions to these problems include antimicrobial peptides produced by all organisms, from bacteria to vertebrates and invertebrates.
We have successfully identified the structure and activity of Lactophoricin (LPcin), an antimicrobial peptide found in bovine milk, through expression using recombinant DNA technology, purification using various biophysical techniques, and structural studies using liquid/solid-state NMR [1, 2]. Based on this LPcin, we constructed a total of 20 analogs library through conservative sequence modification to develop new antimicrobial peptides with shorter length and improved antimicrobial activity than conventional LPcin. Among them, six new antimicrobial peptides were selected through bacterial killing assays and growth inhibition assays, and each peptide was obtained a high purity, sufficient amount for structural studies by optimization of the expression and purification process [3, 4]. In addition, each peptide identified its potential as a new antibiotic through hemolysis assay and cytotoxicity assay, and to investigate the relationship between active-structure by conducting a 3D topology structure study using liquid/solid-state NMR.
 Park TJ., Kim JS., Ahn HC. and Kim Y., “Solution and Solid-State NMR Structural Studies of Antimicrobial Peptides LPcin-I and LPcin-II”, Biophysical Journal, Vol. 101, (2011), pp 1193–1201.
 Jeong JH, Kim JS, Choi SS. and Kim Y., “NMR Structural Studies of Antimicrobial Peptides: LPcin Analogs”, Biophysical Journal, Vol. 110, (2016), pp 423–430.
 Kim JS., Jeong JH. and Kim Y., “Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin” J. Microbiol. Biotechnol. Vol. 27, (2017), 759–767
 Kim Y., Kim JS., Jeong JH., “Design and NMR Structural Studies of new Antimicrobial Peptides with higher activity” Biophysical Journal, Vol. 114, (2018), pp 159a.
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|Notice||Spring semester 2019 Seminar Schedule||-||-|
|58||[April 4. 2019] Rational Design of Solid-State Materials with Asymmetric Coordination Environment||Prof. Kang Min Ok (Sogang University)||Thu. 4 Apr. 2019|
|»||[March 28. 2019] NMR Structural Studies of engineered novel antimicrobial peptides||Prof. Yongae Kim (Hankuk University of Foreign Studies)||Thu. 28 Mar. 2019|
|56||[March 21, 2019] Nanomaterials Design for Energy Conversion and Storage||Prof. Jong-Hyuk Park (Yonsei University)||Thu. 21 Mar. 2019|
|55||[March 27, 2019] Hyperpolarization Researches with NV center, 129Xe and Parahydrogen||Prof. Keunhong Jeong||March 27, 2019|
|54||[March 20, 2019] Challenges in molecular dynamics simulations of unfolded proteins and protein-DNA complexes||Dr. Jejoong Yoo||March 20, 2019|
|53||[March 5. 2019] Innovation@Agilent||Dr. Darlene Solomon (Agilent Technologies Inc.)||Tuesday. 5 March. 2019|
|52||[March 5. 2019] Prof. Michael J. Sailor/Treatment of S. aureus Bacterial Infections with Porous Silicon Nanoparticles||Prof. Michael J. Sailor (UC San Diego)||Tuesday. 5 March. 2019|
|51||[Thu. 6 Dec. 2018] Prof. Dong-Ho Kim/Department of Chemistry, Yonsei University, Seoul, Korea||Prof. Dong-Ho Kim (Yonsei University)||Thu. 6 Dec. 2018|
|50||Fall semester 2018 Seminar Schedule||-||-|
|49||[January 24, 2018] Electro-Optic Organic THz Wave Sources||Prof. O-Pil Kwon(Ajou University)||January 24, 2018 (Wednesday)|
|48||[Thu. 30 Nov. 2017] Prof. Sunmin Ryu/Department of Chemistry, POSTECH, Pohang, South Korea||Prof. Sunmin Ryu||Thu. 30 Nov. 2017|
|47||[Thu. 23 Nov. 2017] Prof. Sung-Gon Kim/Department of Chemistry, Kyonggi University, Suwon, Korea||Prof. Sung-Gon Kim||Thu. 23 Nov. 2017|
|46||[November 14, 2017] Zero-Point Energy, Fluctuations, and Dissipation : A (Mostly) Quantum-Optical Perspective||Prof. Milonni, Peter||November 14, 2017|
|45||[Thu. 16 Nov. 2017] Prof. Hyungjun Kim/Department of Chemistry, and Graduate school of Energy, Environment, Water, and Sustainability (EEWS), KAIST, Daejeon, Korea||Prof. Hyungjun Kim||Thu. 16 Nov. 2017|
|44||[Fri. 30 Oct. 2017] Prof. Igor Rodin/Department of Chemistry, Moscow State University, Moscow, Russia||Prof. Igor Rodin||Fri. 30 Oct. 201|
|43||[October 31, 2017] New ways of vision: Protein structures in translational medicine and business development, my experience||Prof. Robert Huber(1988 Nobel Prize in Chemistry)||October 31, 2017|
|42||[Thu. 26 Oct. 2017] Dr. Jung-Nyoung Heo/Center for Medicinal Chemistry Research, Korea Research Institute of Chemical Technology, Daejeon, Korea||Dr. Jung-Nyoung Heo||Thu. 26 Oct. 2017|
|41||[October 25, 2017] Ultrafast electron microscopy: applications in chemical and materials sciences||Dr. Byung-Kuk Yoo||October 25, 2017|
|40||[Mon. 16 Oct. 2017] Prof. Kirk S. Schanze/Department of Chemistry, University of Texas at San Antonio, Texas, USA||Prof. Kirk S. Schanze||Mon. 16 Oct. 2017|
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