|Speaker||Prof. Yongae Kim (Hankuk University of Foreign Studies)|
|Date||Thu. 28 Mar. 2019|
|Venue||#331, Asan Hall, College of Science|
NMR Structural Studies of engineered novel antimicrobial peptides
Since the discovery of penicillin, antibiotics have brought benefits such as prolonging human life, treating bacterial infections as well as promoting animal growth, but misuse and abuse of antibiotics have caused serious public health problems and economic losses. Thus, antibiotics that work in a completely different way from conventional antibiotics have become necessary, and long-term solutions to these problems include antimicrobial peptides produced by all organisms, from bacteria to vertebrates and invertebrates.
We have successfully identified the structure and activity of Lactophoricin (LPcin), an antimicrobial peptide found in bovine milk, through expression using recombinant DNA technology, purification using various biophysical techniques, and structural studies using liquid/solid-state NMR [1, 2]. Based on this LPcin, we constructed a total of 20 analogs library through conservative sequence modification to develop new antimicrobial peptides with shorter length and improved antimicrobial activity than conventional LPcin. Among them, six new antimicrobial peptides were selected through bacterial killing assays and growth inhibition assays, and each peptide was obtained a high purity, sufficient amount for structural studies by optimization of the expression and purification process [3, 4]. In addition, each peptide identified its potential as a new antibiotic through hemolysis assay and cytotoxicity assay, and to investigate the relationship between active-structure by conducting a 3D topology structure study using liquid/solid-state NMR.
 Park TJ., Kim JS., Ahn HC. and Kim Y., “Solution and Solid-State NMR Structural Studies of Antimicrobial Peptides LPcin-I and LPcin-II”, Biophysical Journal, Vol. 101, (2011), pp 1193–1201.
 Jeong JH, Kim JS, Choi SS. and Kim Y., “NMR Structural Studies of Antimicrobial Peptides: LPcin Analogs”, Biophysical Journal, Vol. 110, (2016), pp 423–430.
 Kim JS., Jeong JH. and Kim Y., “Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin” J. Microbiol. Biotechnol. Vol. 27, (2017), 759–767
 Kim Y., Kim JS., Jeong JH., “Design and NMR Structural Studies of new Antimicrobial Peptides with higher activity” Biophysical Journal, Vol. 114, (2018), pp 159a.
|Notice||Spring semester 2019 Seminar Schedule||-||-|
|68||Fall semester 2016 Seminar Schedule||-||-|
|67||Spring semester 2017 Seminar Schedule||-||-|
|66||[Fri. 17 Mar. 2017] 9th RINS Symposium||-||Fri. 17 Mar. 2017|
|65||[Thu. 27 Apr. 2017] Seminar was canceled!||-||-|
|64||[Wed. 5 July. 2017] Seminar is canceled!||-||-|
|63||7th Jilin-Korea-Waseda Alliance Annual Symposium||-||August 17 ~ 19, 2017|
|62||Fall semester 2017 Seminar Schedule||-||-|
|61||Fall semester 2018 Seminar Schedule||-||-|
|60||[October 10, 2017] Novel Terahertz Spectroscopies||Director Mischa Bonn||Tue, October 10, 2017|
|59||[August 14. 2019] Principle of Semiconductor Quantum Cascade Laser and It’s Application to Chemical Gas Detection||Dr. Byeong-il Seo(Agency for Defense Development)||Wed. 14 August. 2019|
|58||[October 25, 2017] Ultrafast electron microscopy: applications in chemical and materials sciences||Dr. Byung-Kuk Yoo||October 25, 2017|
|57||[March 5. 2019] Innovation@Agilent||Dr. Darlene Solomon (Agilent Technologies Inc.)||Tuesday. 5 March. 2019|
|56||[May 16. 2019] Nano Structure Analysis by Synchrotron Small Angle X-ray Scattering Technique||Dr. Hyungjoo-An (Pohang Accelerator Laboratory)||Thu. 16 May. 2019|
|55||[Fri. 13 Oct. 2017] Dr. J. Nathan Hohman/The Molecular Foundry, Lawrence Berkeley National Laboratory, California, USA||Dr. J. Nathan Hohman||Fri. 13 Oct. 2017|
|54||[March 20, 2019] Challenges in molecular dynamics simulations of unfolded proteins and protein-DNA complexes||Dr. Jejoong Yoo||March 20, 2019|
|53||[Thu. 26 Oct. 2017] Dr. Jung-Nyoung Heo/Center for Medicinal Chemistry Research, Korea Research Institute of Chemical Technology, Daejeon, Korea||Dr. Jung-Nyoung Heo||Thu. 26 Oct. 2017|
|52||[October 15. 2019] A more sustainable chemistry with elemental sulfur||Dr. Thanh Binh Nguyen(Institut of Natural Product Chemistry)||Tue. 15 October. 2019|
|51||[Thu. 13 Apr. 2017] Dr. Wan Ki Bae/Photoelectronic Hybrid Research Center, Korea Institute of Science and Technology, Seoul, Korea||Dr. Wan Ki Bae||Thu. 13 Apr. 2017|
|50||[Mon. 13 Oct. 2016] Prof. Amir H. Hoveyda/Multicomponent Catalytic Synthesis of Organoboron Compounds for Scalable Natural Product Synthesis||Prof. Amir H. Hoveyda||Monday 13 October 2016|
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