|Speaker||Prof. Yongae Kim (Hankuk University of Foreign Studies)|
|Date||Thu. 28 Mar. 2019|
|Venue||#331, Asan Hall, College of Science|
NMR Structural Studies of engineered novel antimicrobial peptides
Since the discovery of penicillin, antibiotics have brought benefits such as prolonging human life, treating bacterial infections as well as promoting animal growth, but misuse and abuse of antibiotics have caused serious public health problems and economic losses. Thus, antibiotics that work in a completely different way from conventional antibiotics have become necessary, and long-term solutions to these problems include antimicrobial peptides produced by all organisms, from bacteria to vertebrates and invertebrates.
We have successfully identified the structure and activity of Lactophoricin (LPcin), an antimicrobial peptide found in bovine milk, through expression using recombinant DNA technology, purification using various biophysical techniques, and structural studies using liquid/solid-state NMR [1, 2]. Based on this LPcin, we constructed a total of 20 analogs library through conservative sequence modification to develop new antimicrobial peptides with shorter length and improved antimicrobial activity than conventional LPcin. Among them, six new antimicrobial peptides were selected through bacterial killing assays and growth inhibition assays, and each peptide was obtained a high purity, sufficient amount for structural studies by optimization of the expression and purification process [3, 4]. In addition, each peptide identified its potential as a new antibiotic through hemolysis assay and cytotoxicity assay, and to investigate the relationship between active-structure by conducting a 3D topology structure study using liquid/solid-state NMR.
 Park TJ., Kim JS., Ahn HC. and Kim Y., “Solution and Solid-State NMR Structural Studies of Antimicrobial Peptides LPcin-I and LPcin-II”, Biophysical Journal, Vol. 101, (2011), pp 1193–1201.
 Jeong JH, Kim JS, Choi SS. and Kim Y., “NMR Structural Studies of Antimicrobial Peptides: LPcin Analogs”, Biophysical Journal, Vol. 110, (2016), pp 423–430.
 Kim JS., Jeong JH. and Kim Y., “Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin” J. Microbiol. Biotechnol. Vol. 27, (2017), 759–767
 Kim Y., Kim JS., Jeong JH., “Design and NMR Structural Studies of new Antimicrobial Peptides with higher activity” Biophysical Journal, Vol. 114, (2018), pp 159a.
|Notice||Spring semester 2019 Seminar Schedule||-||-|
|68||Fall semester 2016 Seminar Schedule||-||-|
|67||Spring semester 2017 Seminar Schedule||-||-|
|66||[Thu. 27 Apr. 2017] Seminar was canceled!||-||-|
|65||[Wed. 5 July. 2017] Seminar is canceled!||-||-|
|64||Fall semester 2017 Seminar Schedule||-||-|
|63||Fall semester 2018 Seminar Schedule||-||-|
|62||7th Jilin-Korea-Waseda Alliance Annual Symposium||-||August 17 ~ 19, 2017|
|61||[Fri. 13 Oct. 2017] Dr. J. Nathan Hohman/The Molecular Foundry, Lawrence Berkeley National Laboratory, California, USA||Dr. J. Nathan Hohman||Fri. 13 Oct. 2017|
|60||[Fri. 16 Jun. 2017] Prof. Martin Thuo/Department of Materials Science and Engineering, Iowa State University, USA||Prof. Martin Thuo||Fri. 16 Jun. 2017|
|59||[Fri. 17 Mar. 2017] 9th RINS Symposium||-||Fri. 17 Mar. 2017|
|58||[Fri. 30 Oct. 2017] Prof. Igor Rodin/Department of Chemistry, Moscow State University, Moscow, Russia||Prof. Igor Rodin||Fri. 30 Oct. 201|
|57||[Fri. 17 Jun. 2016] Prof. Jianwei Sun/Remote Control in Brønsted Acid Catalysis: New Strategies for the Synthesis of Chiral Building Blocks and Medium Rings||Prof. Jianwei Sun||Friday 17 June 2016|
|56||[January 24, 2018] Electro-Optic Organic THz Wave Sources||Prof. O-Pil Kwon(Ajou University)||January 24, 2018 (Wednesday)|
|55||[March 20, 2019] Challenges in molecular dynamics simulations of unfolded proteins and protein-DNA complexes||Dr. Jejoong Yoo||March 20, 2019|
|54||[March 27, 2019] Hyperpolarization Researches with NV center, 129Xe and Parahydrogen||Prof. Keunhong Jeong||March 27, 2019|
|53||[Mon. 16 Oct. 2017] Prof. Kirk S. Schanze/Department of Chemistry, University of Texas at San Antonio, Texas, USA||Prof. Kirk S. Schanze||Mon. 16 Oct. 2017|
|52||[Mon. 9 Jan. 2017] Prof. Sungwook Woo/Wyss Institute for Biologically Inspired Engineering, Harvard University, USA||Prof. Sungwook Woo||Mon. 9 Jan. 2017|
|51||[Mon. 13 Oct. 2016] Prof. Amir H. Hoveyda/Multicomponent Catalytic Synthesis of Organoboron Compounds for Scalable Natural Product Synthesis||Prof. Amir H. Hoveyda||Monday 13 October 2016|
|50||[November 14, 2017] Zero-Point Energy, Fluctuations, and Dissipation : A (Mostly) Quantum-Optical Perspective||Prof. Milonni, Peter||November 14, 2017|
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